As shown in Figures 2D and S2, CD38 CAR-T cells secreted large amounts of TNF-α, IFN-γ, IL-2 and granzyme when incubated with CD38-positive tumor cells compared with pan-T cells, and there was no significant change in the killing effect of CD38 CAR-T cells on tumor cells that did not express CD38. This evidence concerns the gene TNF and neoplasm.