The 11 selected thyroid-specific RNA transcripts (TPO, TG, GFRA2, IYD, PDE8B, WDR86, C16orf89, DGKI, DIO2, TSHR, and PAX8) were amplified from healthy volunteers (Supplementary Figure 2A) and thyroid patients (Supplementary Figure 2B). Here, C16orf89 is linked to thyroid gland disorder.