These results indicate that high expression of GTSE1, TROAP, IQGAP3, KIF23, HJURP, ASPM, MIKI67, KIF20A, FAM64A, and KIF2C might be unfavorable for glioma prognosis, whereas high expression of LDHD, ETNPPL, SCN2B, MRVI1, NEBL, ADARB2, FBXW4, CBX7, LYNX1, and SPOCK2 may be beneficial for the prognosis of glioma. This evidence concerns the gene KIF20A and glioma.