In summary, Rmrp expression was found to be increased in DN and high glucose induced-MC, and the abnormal re-activation of Rmrp was mediated by transcription factor SP1.Furthermore, we found that Rmrp might sponge miR-1a-3p to release JunD expression and activate cell proliferation and fibrosis during DN. This evidence concerns the gene JUND and liver dysplastic nodule.