Furthermore, among the NCI-60 cell lines investigated in this study, the leukemia RPMI-8226 and melanoma SK-MEL-28 cell lines were identified to harbor EGFR mutations which led to their resistance to 12 TKIs, including erlotinib (Ikediobi et al., 2006; Liu et al., 2007). This evidence concerns the gene EGFR and melanoma.