The univariate analysis indicated that higher infiltration levels of CD4+ T cell, macrophages, neutrophils, and dendritic cells were correlated with poor survival outcomes in COAD (Supplementary Figure 1), while low infiltration levels of CD8+ T cell, B cells, macrophages, neutrophils, and dendritic cells were correlated with poor survival outcomes in READ. This evidence concerns the gene CD4 and colon adenocarcinoma.