The knockdown of GPR83 expression was accomplished by administration of GPR83 shRNA lentiviral particles (0.5 μl; 109 particles/μl) into the BLA, CeA, or NAc of female mice (Figures 5A,G,M) and anxiety-related behaviors analyzed using the EPM and open field tests and compared to mice that were administered with control virus. The gene discussed is GPR83; the disease is Anxiety.