In the open field test (Figure 2D) the sex-dependent analysis of anxiety-related behavior revealed that male GPR83 KO mice spent significantly more time in the center compared to wild-type males, an effect that was not seen when comparing female GPR83 KO mice with wild-type females (Figure 2E; two-way ANOVA; interaction F(1,49) = 6.34, p = 0.0150; GPR83 KO F(1,49) = 4.67, p = 0.0355; sex F(1,49) = 0.15, p = 0.7044; Tukey’s post hoc test males: WT vs GPR83 KO, ∗p < 0.05). Here, GPR83 is linked to Anxiety.