This analysis revealed that both estrus and diestrus mice with GPR83 KD in the BLA exhibit significant decreases in time spent in the open arms indicating that the increases in anxiety are not estrus cycle-dependent (Figure 6B; two-way ANOVA; interaction F(1,21) = 0.75, p = 0.3955; GPR83 KD F(1,21) = 22.83, p = 0.0.0001; estrus cycle F(1,21) = 0.01, p = 0.9314; Bonferroni post hoc test, estrus control virus vs GPR83 KD, p < 0.0001; diestrus control virus vs GPR83 KD p < 0.05). This evidence concerns the gene GPR83 and Anxiety.