As shown in Figure 2 and Supplementary Figure S2, immunohistochemistry, western blot, and real-time PCR detected that renal fibrosis such as increased expression of fibronectin, collagen I and collagen IV, the three major fibrosis markers in diabetic kidneys, was increased in WT mice but attenuated in latent TGF-β1 Tg mice. The gene discussed is TGFB1; the disease is renal fibrosis.