Next, glioma cells were isolated from three GBM patients, and patient-derived glioma cells (PD-G) were stimulated into glioma stem cells (PD-GSCs), which were identified by Nestin staining (Fig. S5A) and pluripotent differentiation ability after culture in complete medium containing FBS (Fig. S5B and S5C). The gene discussed is NES; the disease is glioblastoma.