Therefore to determine the potential role of MAP4K4 in pancreatic cancer via MLK3 phosphorylation, stable pancreatic cancer cell lines, Capan-1 and AsPC-1 were created, expressing either doxycycline (DOX)-inducible MLK3 -WT or -T738A (phospho-deficient) or -T738D (phosphomimetic). Here, MAP4K4 is linked to pancreatic neoplasm.