Therefore, the aims of this study were two‐fold: (1) antigen preparation and evaluation of three antigens of GsL3, crude somatic antigen (CSAg), excretory‐secretory antigen (ESAg) and partially purified antigens, namely P1Ag, P2Ag and P3Ag, to differentiate IgE, IgG, IgG1‐4 and IgM for gnathostomiasis diagnosis; and (2) application of the selected ELISA for following up stored sera of patients treated with ivermectin (IVM) and albendazole (ABZ). Here, CD40LG is linked to gnathomiasis.