KRAS and pancreatic neoplasm: Harnessing a previously established model of pancreatic cancer that relies on multiallelic embryonic stem cells that harbour a pancreas-specific Cre driver (p-48 Cre), a conditional mutant Kras allele (LSL-KrasG12D), a conditional reverse tetracycline transactivator allele (Caggs-LSL-rtTA3-IRES-Kate) as well as a homing cassette in the ColA1 locus, we inserted TRE-promoter driven and GFP-labelled shRNAs targeting Kdm6a (shKdm6a) or Renilla luciferase (shRenilla) using recombinase mediated cassette exchange and generated chimeric mice for further experimental use (figure 6A).