IRF8 and chronic myelogenous leukemia, BCR-ABL1 positive: To compare the significance of IRF8 downregulation and that of FLT3 downregulation in cDC development in CML, we transduced a bicistronic retrovirus harboring IRF8 or FLT3 and human CD8t (hCD8t) into mouse BM lineage-negative cells, together with another bicistronic retrovirus encoding BCR-ABL1 and green fluorescent protein (GFP).