To determine the regulatory impact of TRIM24-driven mammary tumorigenesis in a physiologically relevant mouse model, we performed deep sequencing of RNA (RNA-seq) isolated from Trim24COE tumors (n = 6, 3 metaplastic carcinosarcoma and 3 carcinomas), control un-diseased mammary glands from age-matched MMTV-Cre animals (n = 4) and mammary tumors spontaneously generated in either FVB or FVB/129 mice in a non-TRIM24 overexpressing background (n = 3). Here, TRIM24 is linked to breast cancer.