We found no difference in the expression of neuropeptides pro-opiomelanocortin (Pomc), neuropeptide Y (Npy), and agouti-related neuropeptide (Agrp) in the arcuate nucleus (Supplementary Fig 6d), suggesting that obesity resistance in AdNT3-Tg mice may not be mediated through a central mechanism, at least not through a hypothalamic POMC or NPY/AGRP neuron-dependent mechanism. This evidence concerns the gene POMC and obesity due to melanocortin 4 receptor deficiency.