Glioblastoma (GB) is the most common and aggressive primary brain tumour in adult human, and androgens may play a role in its development.Therefore, here, we investigated the expression of four key enzymes involved in androgen synthesis in human‐derived GB cells.Immunocytochemistry and immunofluorescence results revealed that glioblastoma cells express 3β‐HSD, P450c17, 17β‐HSD and 5α‐reductase in their cytoplasm. The gene discussed is CYP17A1; the disease is glioblastoma.