To figure out the role of RanBP3 in CML cells, we used shRNA lentivirus to knockdown RanBP3 in K562 and K562/G01 cells and found that silencing of RanBP3 could significantly inhibit proliferation and induce apoptosis. This evidence concerns the gene RANBP3 and chronic myelogenous leukemia, BCR-ABL1 positive.