Ba/F3-ITD and MV4-11 cells and blood blasts from two patients with AML with FLT3-ITD were cultured for 36 hours with daunorubicin and/or AZD1208, or DMSO control, and levels of PARP1, DNA polymerase θ, DNA ligase 3, XRCC1 and c-Myc protein were measured in whole cell and nuclear lysates (Figure 3A). This evidence concerns the gene LIG3 and acute myeloid leukemia.