TICAM1 and infection: Crystal violet staining showed apparent CPE in the control cells, whereas the CPE was markedly reduced in TRIF-overexpressing cells after SVCV infection (Figure 8A). As shown in Figure 8B, the transcriptional levels of two SVCV genes (SVCV-G and SVCV-N) were significantly attenuated in carp TRIF-overexpressing cells at 3 and 6 h after SVCV infection, which suggest that carp TRIF can inhibit SVCV replication.