IHC analysis of adjacent brain sections with the mHTT aggregate-directed monoclonal antibody mEM48 (Supplementary Fig. S5A) and determination of aggregated HTT levels in brain homogenates by the aggregate-specific MSD assay (MW8/4C9-ST; Supplementary Fig. S5B, right) similarly revealed the age-dependent increase of HTT aggregates in the HOM zQ175 HD mouse model (Fig. 5B). Here, HTT is linked to Huntington disease.