HTT and Huntington disease: As previously demonstrated47–50 for HD mouse models and expanded upon in this report, we observed an age- (or disease-) dependent increase in brain (hippocampus, cortex and striatum) aggregate load (density and size) in progressively increasing ages of HOM zQ175 and R6/2 mice using immunochemistry with mEM48 antibody (Fig. 5B, C); no significant immunoreactive signal was detected at any age from age-matched wild-type controls, as these mice do not express mHTT nor HTT aggregates (Supplementary Fig. S5A).