WT1 and cancer: To further investigate the anti-cancer contribution of WT1 targeting by miR-642a-5p, we transiently and stably overexpressed WT1 cDNA (WT1-203 isoform) in 22Rv1 and LNCaP PCa cells, and transfected these cells with 30 nM miR-642a-5p or miR-NC.