We also performed flow cytometry cell cycle analysis of 22Rv1 and LNCaP PCa cells transfected with WT1 siRNA or si-NC, and found that WT1 knockdown induced cell cycle arrest at G0/G1, and a concurrent increase in p21 and p53 expression (Fig. 5d, e). The gene discussed is TP53; the disease is posterior cortical atrophy.