To ascertain whether WT1 is a direct target of miR-642a-5p, we transiently co-transfected a luciferase reporter construct containing the first 1293 base pairs (bp) of WT1’s 3′UTR region (total length 5146 bp) (Fig. 4b), along with miR-642a-5p or miR-NC into 22Rv1 and LNCaP PCa cells. Here, WT1 is linked to posterior cortical atrophy.