In addition, we also performed transwell assays with other types of cancer cells, such as breast cancer (Hs 578t), hepatocellular carcinoma (PLC/PRF/5), thyroid cancer (FTC-133), and esophageal cancer (KYSE-150), transfected with si-NSUN2, which contributed inconsistent results (Fig. S1). The gene discussed is NSUN2; the disease is esophageal cancer.