AKT1 and breast cancer: To ask whether Uev1A regulates CT45A expression through the AKT pathway in breast cancer cells, phosphorylation levels of both AKT-Thr308 and AKT-Ser473 in MDA-MB-231 and MCF7 cells transiently overexpressing UEV1A were first monitored by western blot and found to be increased (Fig. 7a).