To ask whether UEV1A overexpression also increases CT45A expression in other cancer cells, we transiently transfected UEV1A in HCT116 colorectal cancer cell lines (Fig. 5a), in which CT45A was moderately upregulated upon UEV1A ectopic expression, but not in UEV1A-F38E-expressed HCT116 cells (Fig. 5b). Here, UBE2V1 is linked to cancer.