In a reverse expreriment, depletion of Uev1A in the above three cancer cell lines significantly inhibited the upregulation of CT45A, indicating that Uev1A plays a critical role in the upregulation of CT45A. Siminarly, Uev1A positively regulates the expression of CT45A downstream tumorigenic, EMT, stemness and metastatis related genes in breast cancer cells. The gene discussed is UBE2V1; the disease is breast cancer.