Collectively, we concluded that knock-down of LncRNA MALAT1 suppressed NLRP3-mediated pyroptotic cell death via releasing miRNA-135b-5p, resulting in the survival of HK-2 cells and targeting the LncRNA MALAT1/ miRNA-135b-5p axis could be used as potential strategy for AKI associated CKD diagnosis and treatment. The gene discussed is NLRP3; the disease is acute kidney injury.