Moreover, consistent with the existed data that cell pyroptosis occurs during the pathogenesis of CKD [44,45] and LPS treatment [46], we evidenced that LPS also triggered NLRP3-mediated pyroptotic cell death in HK-2 cells, suggesting that cell pyroptosis was closely associated with LPS-induced AKI development. The gene discussed is NLRP3; the disease is chronic kidney disease.