S1PR1 and urinary bladder cancer: To understand the direct effect of S1PR1 expression on bladder cancer cells, 21 bladder cancer-related cell lines were screened from the CCLE database for S1PR1 expression analysis, showing that the high S1PR1 expression group generally had lower methylation and the low S1PR1 expression group had higher methylation in addition to lower copy number variation (Figure 5A).