The performance of R-117bp assay (3.5 mM MgCl2 and 58.2 °C) was evaluated using a first set as follows: 5 serum pools (each consisting of 5 samples) from healthy donors, 5 serum pools (each consisting of 5 samples) from PCa patients with increasing average PSA values (12.8 to 602.2 ng/mL) (summarized in Table S1). This evidence concerns the gene KLK3 and posterior cortical atrophy.