The presence of NRDP1 in the nucleus of all four CaP cells was validated by subcellular fractionation studies (Figure 1B, full blot; Figure S6), which revealed that CRPC cell lines (PC3 and CWR22Rv1) express higher levels of nuclear NRDP1 compared to androgen sensitive cell lines (LNCaP and MDA-PCa-2b); cytoplasmic to nuclear ratio of NRDP1 was 1:0.57 in MDA-PCa-2b cells and 1:1.23 in LNCaP cells compared to 1:10.05 in CWR22Rv1 cells and 1:3.28 in PC3 cells (Figure 1B). Here, RNF41 is linked to posterior cortical atrophy.