To test our automated PD-L1 scoring, three different PD-L1 scores were determined for 54 cases of HNSCC based on PD-L1 assessment manually by the above mentioned four human investigators (T.B., S.K., F.S., B.P.)and automated by machine (Figure 3A–C, in pseudo-log scale for better visualization of low scores see Figure S1A–C). This evidence concerns the gene CD274 and head and neck squamous cell carcinoma.