TSC2 and hepatocellular carcinoma: Similarly, in a larger cohort study enrolling 121 advanced HCC patients, mutation profiling of ctDNA revealed mutations in the most frequent HCC-associated driver oncogenes and tumor suppressors, including the TERT promoter, TP53, CTNNB1, PTEN, AXIN1, ARID2, KMT2D and TSC2.