As reported, LNCaP, C4-2, 22RV1, and VCaP cells exhibited robust AR activity by means of KLK3 expression, whereas PC3 and DU145 (AR-negative prostate cancer cells) or RWPE1, PWR1E, and BPH1 cells (benign prostate immortalized cells) exhibited low activity of the nuclear receptor (Figure 2A). This evidence concerns the gene AR and Familial prostate cancer.