In addition, genes with a significant dependency score in 50% of the tested NSCLC cell lines in CRISPR-Cas9 (n = 78) or RNAi (n = 92) were defined as layer 1 proliferation genes, including URM1, VIRMA, BUB3, POLA1, NDUFS8, OR4K1, and MYC (Figure 4A). The gene discussed is POLA1; the disease is non-small cell lung carcinoma.