When fitting these 33 genes in a multivariate Cox model in a separate NSCLC patient cohort GSE81089 (n = 197), five significant (p < 0.05, multivariate Cox modeling) genes, including EIF2AK3, F2RL3, FOSL1, SLC25A26, and SPP1, were selected as layer 2 prognostic genes. This evidence concerns the gene F2RL3 and non-small cell lung carcinoma.