From the list of the layer 2 candidate genes, 13 genes (COPA, CSE1L, EIF2B3, LSM3, MCM5, PMPCB, POLR1B, POLR2F, PSMC3, PSMD11, RPL32, RPS18, and SNRPE) had a significant dependency score in 100% of the tested NSCLC cell lines in both CRISPR-Cas9 (n = 78) and RNAi assays (n = 92), defined as layer 2 proliferation genes. This evidence concerns the gene COPA and non-small cell lung carcinoma.