The identified NSCLC proliferation genes (COPA, CSE1L, EIF2B3, LSM3, MCM5, PMPCB, POLR1B, POLR2F, PSMC3, PSMD11, RPL32, RPS18, and SNRPE) had a significant impact on 100% of NSCLC cell lines in CRISPR-Cas9 and RNAi assays. Here, RPS18 is linked to non-small cell lung carcinoma.