We engineered the Ad5 genome DNA using the MUC16/1040-bp fragment to replace the E1A promoter to control E1A expression, as illustrated in Figure 2A. In the E3 region, we inserted a fusion gene, TK-EGFP, with both EGFP activity that can be used to track virus infection and herpes simplex virus-1 thymidine kinase (HSV-TK) activity that can convert pro-drug, ganciclovir (GCV), or other analogs, into a toxic metabolite to kill host cells [22,23]. This evidence concerns the gene DHTKD1 and viral infectious disease.