As shown in Figure 1C, the MUC16-1040 fragment showed equivalent or slightly higher activity in HeLa cells compared to the E1A promoter, but much lower activity (27.3%) in the A549 lung adenocarcinoma cell line, which is characterized by ease of use for adenovirus amplification and low expression of CA-125. The gene discussed is MUC16; the disease is lung adenocarcinoma.