Four CpGs sites were at the ADIPOQ locus had different methylation levels (two in visceral adipose tissue and two in maternal blood) in the groups, but only the R3 CpG1 site in visceral adipose tissue, which showed greater methylation in GDM, inversely correlated with mRNA levels of adiponectin. This study highlights the importance of the two types of adipose tissue in the pathophysiology of GDM. Here, ADIPOQ is linked to gestational diabetes.