The increased stiffness of SCECs in glaucoma could be associated with the increased expression of cell adhesion marker VE-cadherin, as well as increased expression of ECM fibrotic markers α-SMA, collagen I-α1 and fibronectin, driven by the pro-fibrotic marker TGF-β2, as observed in this in vitro study. The gene discussed is ACTA1; the disease is glaucoma.