Then, the PKCα-specific peptide was reacted with lysates of human bladder cancer cells [KU-1 (grade 2), KU-7 (grade 1), T24 (grade 3), TCCSUP (grade 4), or UMUC-3 (grade 3)], tissues from UMUC-3-bearing mice, or normal skin tissues. This evidence concerns the gene PRKCA and urinary bladder cancer.