For GLUT1, 83.33% (25/30) of ESCC samples show intermediate to high positive intensity, only 16.67% (5/30) of ESCC tissue show low to negative positivity, compared to normal esophagus tissue, which shows 100% (27/27) low-to-negative GLUT1 expression (p < 0.001). This evidence concerns the gene SLC2A1 and esophageal squamous cell carcinoma.