We calculated the sensitivity (the percentage of 2-DG 800CW positive area in GLUT1 positive area) and specificity (the percentage of 2-DG 800CW negative area in GLUT1 negative area) of 2-DG 800CW in detection of GLUT1 overexpression on 4 μm slices of HGD and ESCC biopsies, resulting in a median sensitivity of 23.02% and a median specificity of 85.43% (n = 19) (see Figure 4c). The gene discussed is SLC2A1; the disease is esophageal squamous cell carcinoma.