To examine whether ATG101 influences cancer growth and survival via induction of autophagy, we first conducted assays comparing proliferation rates of MIA PaCa-2 pancreatic cancer cells between sgControl (irrelevant sgRNA)-transfected cells and corresponding sgATG101-transfected ATG101 knockout (KO) cells generated using the Lenti-CRISPR system. Here, ATG101 is linked to cancer.