Immunoblot analysis revealed more accumulated levels of p62 and cytoplasmic LC3-I form than the levels of autophagosome-bound LC3-II forms (lower levels of the ratio LC3-II/I) in ATG101 KO cancer cells compared to corresponding sgCTL cells under both nutrient complete and amino acid-deprived conditions (–AA) (Figure 1c). Here, ATG101 is linked to cancer.