Moreover, we also investigated the physical interaction between ATG101 and HUWE1 using co-IP and found that endogenous HUWE1 co-immunoprecipitated with endogenous ATG101 in MIA PaCa-2 pancreatic cancer cells as well as with ectopically expressed FLAG-ATG101 in HEK293T cells using a primary antibody against HUWE1, confirming a physical association between ATG101 and HUWE1 in basal conditions (Figure 3d,f). This evidence concerns the gene HUWE1 and familial pancreatic carcinoma.