The ten 9-mer peptides yielding the highest predictive scores for HLA-binding, retrieved by the three different bioinformatics algorithms, were therefore evaluated in an 8-day culture setting in which CD8+ T cells obtained from peripheral blood (PB) of healthy subjects and AML patients, were ex vivo stimulated with antigen-presenting cells (APCs) pulsed with each individual peptide, and tested for cytokine secretion capability. Here, CD8A is linked to acute myeloid leukemia.