T cells transduced with the transgenic NPM1-mutated neoantigen TCR were functional, resulting in specific recognition and release of IFNγ upon incubation with HLA-A*02:01-positive T2 cells loaded with CLAVEEVSL peptide, as well as with OCI-AML3 cell line and HLA-A*02:01-positive primary AML blasts harboring NPM1 mutation. The gene discussed is NPM1; the disease is acute myeloid leukemia.