CAR-T cells exhibited potent in vitro cytotoxicity against HLA-A2-positive cell lines and primary blast cells expressing NPM1-mutated epitopes, but not against HLA-A2-positive leukemic cells without NPM1-mutated expression and different tumor cells in the absence of HLA-A2 expression, supporting the specificity of NPM1-mutated CAR-T cell recognition and the killing of target cells with AIQ-HLA-A2 complex on the cell surface. The gene discussed is NPM1; the disease is neoplasm.