Prior to the injection of the NDEVs into the mouse brain, we also demonstrated that the NDEVs had the requisite exosome-related tetraspanin markers, that DS-AD NDEVs were able to elicit the amplification of seeding competent p-Tau, and that the average particle size was comparable to what has been reported for exosomes in the literature [90]. The gene discussed is MAPT; the disease is Alzheimer disease.