The present study aimed to investigate the anti-inflammatory effects of the Artemisia anomala S. Moore ethanol extract (EAA) by inhibiting tumor necrosis factor-α/interferon-γ (TNF-α/IFN-γ)-induced ERK and NFκB signaling in HaCaT cells, and by improving the skin conditions in 2, 4-dinitrochlorobenzene (DNCB)-induced atopic dermatitis-like lesions in the mouse model. The gene discussed is NFKB1; the disease is atopic eczema.