TTBK2 and ciliopathy: We further confirmed these findings in the context of full-length proteins in a pull-down experiment with HA-tagged TTBK2 and FLAG-tagged CEP164, WT, Y73A, S82A, W84, and Q11P mutants (Figure S1B) that corroborated a strong (Y73A and W84A) or a significant reduction (ciliopathy mutation Q11P) of the TTBK2-CEP164 interaction, while no binding defect was observed for the S82A mutant.