SHBG and obesity due to melanocortin 4 receptor deficiency: In this 5–10 year follow-up of the PregMet-study, we assessed adrenal, gonadal and adipose tissue steroid hormones related to PCOS, in addition to hepatic sex-hormone-binding globulin (SHBG), which is inversely associated with obesity and insulin resistance; and calculated free testosterone [27–29].