TGFB1 and metastatic malignant neoplasm: To check the EMT activities of the primary and metastatic cancer cells, the transcriptional levels of canonical EMT-related signature genes, including epithelial feature genes (CDH1, KRT8, KRT18, CTNNB1, and ZEB1), mesenchymal feature genes (VIM, S100A4, FN1, and LAMA5), and EMT signature genes (SNAI1, SNAI2, ITGB6, TGFB1, and TGFB2) were visualized and compared between primary and metastatic cancer cells (Figure 7A).