As shown, we found that higher tRF-31-U5YKFN8DYDZDD expression was significantly associated with depth of tumor invasion (P = 0.016), lymph node metastasis (P = 0.010), higher TNM stage (P = 0.003), and positive vascular invasion (P = 0.033), but no significant relationship with age, gender, differentiation grade, tumor size, Lauren classification, nerve invasion, and the expression of C-erbB-2, CEA, CA199, CA724. Here, CEACAM5 is linked to neoplasm.