In order to declare the role and underlying mechanism of lncRNA in GC, this study used high-throughput microarray to compare and determine the differences of lncRNA expression profiles between tumor and adjacent tissues, and uncover the role and mechanism of lncRNA PITPNA-AS1 in the progression of GC through multiple screening strategies, clinical sample analysis and functional loss assay. The gene discussed is PITPNA; the disease is neoplasm.