MAP1LC3A and osteosarcoma: The results showed that the average number of GFP-LC3 puncta per cell was 4.6, 23.4, 32.2, 35, 37.2 and 42.2, which suggested that treatment of osteosarcoma cells with doxycycline resulted in a significant increase in GFP-LC3 puncta formation (Figure 5).