Overall, the approach allowed for a comprehensive analysis of changes in marker expression on gluten‐specific CD4+ T cells upon gluten challenge, and the results were contrasted with marker expression in gluten‐specific blood T cells of untreated CeD patients, i.e., patients consuming gluten, and with that of the CeD associated (CD38+ CD103+) CD8+ and γδ+ T cells. The gene discussed is CD8A; the disease is cranioectodermal dysplasia.