The removal of Nhlh2 from Kiss1 neurons did not induce any metabolic phenotype in Kiss1Cre:Nhlh2fl/fl mice under regular or HFD chow, which supports a role for Nhlh2 in POMC neurons in the obesity phenotype of whole-body KOs. Here, KISS1 is linked to obesity due to melanocortin 4 receptor deficiency.