CD274 and cancer: established HEK293T cells to stably express the mouse PD‐1 receptor on the cell membrane to enhance the cancer immunotherapy through disrupting the PD‐1/PD‐L1 immune inhibitory axis.[42c] (Figure 5A–C) Just like the source cells, the PD‐1 preserved right side out orientation in following PD‐1 displaying Bio‐MVs, which is crucial for the effective blockade of PD‐1/PD‐L1 connection.