successfully engineered a kind of Bio‐MVs from a model cancer cell line to express the co‐stimulatory molecule CD80 and enable it to present its antigens in immune‐costimulatory content with CD28 on T‐cells.[106] (Figure 6) They selected the B16‐F10 murine melanoma cell line as a research object because of their inherent expression of MHC I, which can present peptide epitopes from the endogenous cellular antigen.[107] The cell line with co‐stimulatory molecule CD80 overexpression was successfully established via transfection with a plasmid encoding CD80 genetic sequence. This evidence concerns the gene CD80 and melanoma.