ALK and neoplasm: RNAscope in situ hybridizations in a MYCN-amplified neuroblastoma case (K10, Supplementary Data 1) as independently confirmed by comparative genomic hybridization (CGH)20, revealed a high intratumoral heterogeneity for TH, MYCN, and ALK expression, with tumor regions with intense staining of MYCN and ALK, negative for TH (Fig. 4a, zoom 2).