Having established that PTEN can suppress PI3K/AKT pathway in a wing growth assay, we proceeded to screen the relative function of PTEN variants from individuals with PHTS, somatic cancer, or ASD (S1 and S2 Tables), using the omb>PI3Kact wing size assay, in addition to PTEN-WT, attP2 (no PTEN) control, and the panel of biochemical variants (C124S, G129E, Y138L, 4A). The gene discussed is AKT1; the disease is PTEN hamartoma tumor syndrome.